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KMID : 0379520080240010029
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한국독성학회지
2008 Volume.24 No. 1 p.29 ~ p.36
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The Effects of Crinum asiaticum on the Apoptosis Induction and the Reversal of Multidrug Resistance in HL-60/MX2
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Hyun Jae-Hee
Kang Ji-Hoon
Park Deok-Bae
Lee Young-Jae
Kim Sang-Cheol
Yoo Eun-Sook
Kim Elvira
Kang Jung-Il
Kang Hee-Kyoung
Kwon Jung-Mi
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Abstract
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The present study investigated the anti-proliferative and chemosensitizing effects of Crinum asiaticum var. japonicum against multi-drug resistant (MDR) cancer cells. The 80% methanol extract, chloroform (CHCl₃) fraction and butanol (BuOH) fraction of C. asiaticum inhibited the growth of mitoxantrone (MX) resistant HL-60 (HL-60/MX2) cells. When HL-60/MX2 cells were treated with the CHCl₃ and BuOH fractions, DNA ladder and sub-G1 hypodiploid cells were observed. Furthermore, the fractions reduced Bcl-2 mRNA levels, whereas Bax mRNA levels were increased. These results suggest that the inhibitory effect of C. asiaticum on the growth of the HL-60/MX2 cells might arise from the induction of apoptosis. Treatment of HL-60/MX2 cells with the fractions markedly decreased the mRNA levels of the multi-drug resistance protein-1 and breast cancer resistance protein. The CHCl₃ fraction and hexane fraction increased MX accumulation in HL-60/MX2 cells. These results imply that the CHCl₃ fraction of C. asiaticum plays a pivotal role as a chemosensitizer. We suggest that components of C. asiaticum might have a therapeutic potential for the treatment of MDR leukemia.
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KEYWORD
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HL-60/MX2, Crinum asiaticum, Apoptosis, Chemosensitizer, MRP-1, BCRP
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